Soil contains a various number of microorganisms in it. Some
of these microorganisms that reside in soil are harmful, while some are
useful. The present investigation was carried out to isolate and
characterization of bacteria from polluted soil samples. Selected bacterial
isolates were evaluated by morphological, and biochemical test. Gram
staining of the bacterial isolates was also performed. Based on Gram’s
staining, 5 bacterial isolates were Gram Positive, while the remaining 3
isolates were Gram Negative. According to Biochemical test (Urease
test, Starch Hydrolysis test, Catalase test) done on isolates, Isolate 3
and Isolate 6 showed Negative on all the tests, while Isolate 7 showed
positive result only on catalase test. Isolate 1, Isolate 2, Isolate 4, and
Isolate 5 showed negative result only on Urease test. While Isolate 8
showed negative result only on catalase test. Out of eight selected
bacterial isolates, only Isolate 8 showed positive for Urease test.
Antibiotic sensitivity test also showed significant result for the selected
isolates. The isolated bacterial strains will be helpful for possible
application of bioremediation of contaminated sites.